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Applications and Articles Applications and Articles


Enhancing HPLC Field Service with fast-response, non-invasive flowmeters

Field Service Engineers are crucial to scientists striving to get the most from their analytical equipment. The maintenance, performance assurance and validation of HPLC/uHPLC systems gains particular importance when this equipment is an essential component in labs subject to compliance found in the pharmaceutical, food, beverage, and environmental analysis sectors.
Published in Lab Asia – June 2024
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Validating HPLC results obtained using any solvent

High-Performance Liquid Chromatography (HPLC) stands as a cornerstone technique in analytical chemistry, facilitating the separation, identification, and quantification of chemical and biological compounds. While many applications are based upon aqueous eluents, HPLC separation protocols also often involve the utilization of other solvents to accommodate the unique properties of different analytes. Ensuring consistent elution profiles is a key requirement to ensure reproducible results. Continuous monitoring of the delivered HPLC eluent flow rate is therefore essential when permanent validation of results is desired or necessary as it is in regulated industries.
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Preventing the ‘Jetstream effect’ in HPLC flow measurements

Determination of flow rate and comparison of the obtained results with expected or nominal values, is a very common task in liquid chromatography. It is also very often part of the validation of an HPLC/UHPLC system, as commonly required by labs following OQ/PQ guidelines. Among the methods available, modern non-invasive flowmeters are without a doubt the most flexible and capable tool for the task, as by nature they can deliver flow rate data with high accuracy and resolution in real time thus providing additional information as pulsation or short-term instability. As such, careful selection of a flow device used for the measurement is paramount for exploiting the full possibilities offered by such a powerful tool.
Published in MicroBioz India – May 2024
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HPLC Gradient Validation using Non-invasive Flowmeters

Gradient HPLC serves as a crucial tool for analysing samples containing compounds with varying polarity. Its effectiveness stems from the ability to tailor both gradient time and composition, enabling the manipulation of analyte retention times. However, challenges arise from deviations in the programmed gradient profiles produced by HPLC systems, impacting retention time and peak shape. Several factors contribute to this variability in gradient HPLC, such as solvent quality, pump performance, changes in system pressure, and the condition of the columns. Addressing these challenges requires precise control and monitoring of gradient profiles.
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Enhancing HPLC Field Service with fast-response, non-invasive flowmeters

Field Service Engineers are crucial to scientists striving to get the most from their analytical equipment. The maintenance, performance assurance and validation of HPLC/UHPLC systems gains particular importance when this equipment is an essential component in labs subject to compliance found in the pharmaceutical, food, beverage, and environmental analysis sectors.
Published in MicroBioz India – April 2024
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Mejora del servicio de campo de HPLC con caudalímetros no invasivos y de respuesta rápida

Los ingenieros de servicio de campo son cruciales para los científicos que se esfuerzan por aprovechar al máximo sus equipos analíticos. El mantenimiento, aseguramiento del rendimiento y validación de los sistemas HPLC/UHPLC cobra especial importancia cuando estos equipos son un componente esencial en laboratorios sujetos a cumplimiento en los sectores farmacéutico.
Published in Laboratorio – April 2024
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Preventing the 'Jetstream effect' in HPLC flow measurements

The flow profile delivered by an HPLC system can be affected by an abrupt change in the internal diameter (ID) of tubing when the change is from a narrow bore to a significantly larger bore. Such an abrupt change in ID causes a "core stream" to be built in the larger ID tubing leaving the liquid layers closer to the wall of the tube practically unperturbed. This 'Jetstream effect' will decisively affect the velocity profile of eluant flow creating disturbances which will decrease the accuracy of HPLC flow rate measurement. Often underestimated, this phenomenon might also affect peak symmetry, it is therefore crucial to understand it and prevent.
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Laboratory 4.0 & Liquid Chromatography

In the world of analytical chemistry, liquid chromatography stands as a cornerstone technique, enabling scientists to separate, identify, and quantify components with unparalleled precision.
Published in MicroBioz India – February 2024
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Les partenariats technologiques favorisent l’innovation dans le domaine de la chromatographie liquide

Le développement de nouveaux produits visant à améliorer les capacités, les performances, la fiabilité, la facilité d’utilisation, la traçabilité des données et le service après‑vente est un objectif stratégique clé pour de nombreuses entreprises d’instrumentation.
Published in La Gazette du Laboratoire – February 2024
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Conformity or Calibration - which certification do you require for your Flowmeter?

All TESTA Analytical Flowmeters come with a Certificate of Conformity which demonstrates compliance of each individual product with our published specifications. The certificate of conformity testifies the internal process involved in assuring a certain quality of each and every flowmeter shipped to clients. This process includes fulfillment of a documented production process to a set of measurements aimed to prove that each and every instrument delivered will perform within specification.
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Technology partnering enables liquid chromatography innovation

Carlo Dessy is Managing Director of Testa Analytical Solutions eK, a respected international OEM developer and supplier of instruments, innovative detectors and software for liquid flow applications. Here I am sharing an interesting interaction with Carlo Dessy.
Published in MicroBioz India – November 2023
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Comparing techniques for flow rate measurement in Liquid Chromatography

Accurate determination of flow rate is one of the most common, and often overlooked, means of assessing or validating the performance of a pump serving a HPLC, UPLC, Ion-Chromatography or GPC/SEC system. Using the right technique, it is nowadays possible to use flow rate monitoring as a powerful diagnostic tool, allowing fast detection of leaks, faulty check-valves and worn seals.
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Studying the Impact of Dissolved and Undissolved Gas on the Performance of Peristaltic Pumps

A set of experiments illustrating how dissolved gas can affect peristaltic pumps in chromatography processes
Published in Lab Manager – July 2023
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Gefahr erkannt, Gefahr gebannt

Der Feind peristaltischer Pumpen sind Gasblasen. Welchen Einfluss gelöstes und ungelöstes Gas auf die Leistung dieser Pumpen hat und damit auf HPLC, GPC & Co. und was sich dagegen unternehmen lässt, erfahren Sie in diesem Beitrag.
Published in Laborpraxis – May-June 2023
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Studying the Impact of Dissolved and Undissolved Gas on the Performance of Peristaltic Pumps

Peristaltic pumps are fluid delivery tools widely used in laboratory techniques including HPLC, GPC/SEC, Flow Chemistry and Liquid Dosing. It is widely acknowledged that dissolved gas can have an impact on the performance of peristaltic pumps. A common solution to address this problem with HPLC systems, is to utilise a vacuum degasser on the inlet side of the pump.
Published in International LabMate – June 2023
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Creating a better differential viscometer

Used as a GPC/SEC detector to determine the specific viscosity of a polymer or protein in solution - differential viscometers have been around for decades and have demonstrated their value in numerous applications. The R&D team at TESTA Analytical has improved the original 4-capillary bridge design and created a new generation differential viscometer with exceptional performance and operational advantages.
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The importance of real time flow monitoring in Analytical labs

Backed by over 30 years of experience in the field of instrument development and device engineering, TESTA Analytical is today a leading developer and supplier of optimised detectors and instruments for liquid chromatography and flow monitoring in particular.
Published in MicroBioz India – February 2023
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Investigating Variation in Flow from Peristaltic Pumps

With peristaltic pumps, several flow paths can be operated simultaneously. However, many parameters influence the actual flow rate in each channel. Accurate determination of the individual flow rate in the individual channels is crucial for reproducible results.
Published in LAB Worldwide – February 2022
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Investigating Variation in Flow from Peristaltic Pumps

Peristaltic pumps find wide application in modern labs, they are considered inexpensive, easy to use and to maintain and, most important of all, are extremely reliable and flexible. The range of application is huge, from simple transfer of a liquid from one container to another in HPLC and Ion chromatographs, to more complex usage as dispensing tools for reagents in flow chemistry.
Published in MicroBioz India – June 2022
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The Importance of Understanding Pump Flow

Pumps are one of the most common tools enabling liquids to be moved, delivered, pushed through a separation column, supplied as a dose or transferred from one container to another. Several different pumping technologies are available to choose from, depending on your application or problem. As you might expect - a pump designed to pump crude oil through a pipeline has very different requirements compared to a pump designed to deliver a constant stream of medication into a patient's vein. As such, the term ‘flow’ can be interpreted very differently, depending on the context.
Published in International LabMate – July 2022
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Alles stabil und im Flow?

Mithilfe von peristaltischen Pumpen lassen sich mehrere Förderwege gleichzeitig betreiben. Doch viele Parameter beeinflussen die tatsächliche Durchflussrate in jedem Kanal. Die genaue Bestimmung des individuellen Durchflusses ist für reproduzierbare Ergebnisse entscheidend.
Veröffentlicht in Laborpraxis – July-August 2022
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Real-Time Monitoring of HPLC Pump Performance

High Performance Liquid Chromatography (HPLC) is nowadays one of the most widely used techniques in analytical chemistry. The range of applications soluble using HPLC covers a vast range of sample types, concentrations, and compositions. This extreme flexibility is achieved by using specific columns carefully designed for each particular investigation...Read More

How Enhanced Detector Stability Leads to Improved GPC/SEC Results

GPC (Gel Permeation Chromatography) or SEC (Size-Exclusion-Chromatography is widely acknowledged as one of the most useful characterization methods in polymer science. The technique offers the possibility to determine molecular weight distribution and its averages, to provide a complete picture of the polymer sample under investigation...Read More

High Performance GPC/SEC System

Gel-permeation chromatography (GPC) coupled with Multi-Angle Static Light Scattering (SLS) is a modern and powerful technique for determining absolute molecular weight values without the need to rely on traditional column calibration... Read More

Why is Flow Rate Important in GPC/SEC?

To obtain reliable data from your GPC/SEC instrument - each run should be related to a calibration curve obtained by measuring appropriate chromatographic standards. Calculations from GPC/SEC experiments are based on elution volume, which is a product of time and flow rate...Read More

Common detectors for GPC/SEC Chromatography

This on-line presentation provides an overview about the detectors most commonly used in GPC/SEC applications, explains differencies... See Presentation